Journal: Cancer Research

Article Title: MCT-1 Protein Interacts with the Cap Complex and Modulates Messenger RNA Translational Profiles

doi: 10.1158/0008-5472.can-06-1999

Figure Lengend Snippet: Figure 3. eIF4E is required for MCT-1 interaction with the cap complex. The eIF4E was immunodepleted from the reticulocyte lysate containing the MCT-1 and MCT-1 deletion proteins. A, Western blot with eIF4E antibody of the lysate before and after eIF4E depletion as well as the depleted eIF4E. B, the cap binding assay was done as in Fig. 2B, but also with the eIF4E-depleted extract (left). Right, 5% of the input MCT-1 and deletion proteins. C, MCT-1 and DENR sediment with the translation initiation complex. Transiently transfected 293HEK cells were used for polysome preparation as described. Fractions 1 to 3 are typically completely devoid of ribosome components and contain multifactor complex. Representative polysome distribution profile (top). Representative Western blot analysis with antibodies against eIF2a, eIF4E, Myc-DENR, V5-MCT-1, h-actin, and rS6p (bottom).

Article Snippet: Half of this reaction (50 AL) was used for overnight depletion of eIF4E by using 4 Ag of eIF4E antibody (Santa Cruz Biotechnology), 20 AL of protein A/G agarose beads and protease inhibitor.

Techniques: Western Blot, Binding Assay, Transfection

Journal: Cancer Research

Article Title: MCT-1 Protein Interacts with the Cap Complex and Modulates Messenger RNA Translational Profiles

doi: 10.1158/0008-5472.can-06-1999

Figure Lengend Snippet: Figure 6. Proposed model for role of MCT-1 in translation. MCT-1 bound to DENR binds to the cap complex either directly or indirectly through interaction with eIF4E with enhanced translation initiation by scanning and recognition of the initiation codon. MCT-1 and DENR might also recruit additional translation factors to the translation initiation complex.

Article Snippet: Half of this reaction (50 AL) was used for overnight depletion of eIF4E by using 4 Ag of eIF4E antibody (Santa Cruz Biotechnology), 20 AL of protein A/G agarose beads and protease inhibitor.

Techniques:

Journal: International Journal of Biological Sciences

Article Title: Cdh1 functions as an oncogene by inducing self-renewal of lung cancer stem-like cells via oncogenic pathways

doi: 10.7150/ijbs.38672

Figure Lengend Snippet: Primers for RT-qPCR

Article Snippet: The following primary antibodies were used: anti-E-cadherin (Proteintech, USA), anti-PI3K, anti-Akt, anti-mTOR, anti-S6K, anti-Eif4e, anti-Erk1/2, anti-p-Mek1/2, anti-p-Erk1/2, anti-p-Akt, anti-p-mTOR, anti-p-S6k (Cell Signaling Technology, USA), anti-Mek1 (Upstate, USA) and anti-GAPDH (Proteintech, USA).

Techniques:

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: 1:100 n.a Thermo EIF4E Mo Thermo MA1–089 AB_2536738 1:100 1:2000 EIF4E2 Mo Santa Cruz sc-100731 AB_1122491 1:100 n/a EIF4E3 Rab ProteinTech 17282–1-AP AB_2262162 1:100 n/a EIF4A1 Mo Thermo MA5–14901 AB_11006110 1:100 n/a EIF4A2 Rab ProteinTech 11280–1-AP AB_2097378 1:100 1:2000 EIF4A3 Rab Abcam Ab32485 AB_732124 1:50 n/a SYCP3 Rat Handel Lab. Eaker et al.,2001 n.a.

Techniques:

Journal: Biology of Reproduction

Article Title: Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice †

doi: 10.1093/biolre/iox150

Figure Lengend Snippet: Some translational factors are relatively enriched in the XY body of pachytene spermatocytes. (A-D) EIF4F complex members EIF4A1, EIF4E, EIF4E2, and phosphorylated EIF4E were found in the XY body. (E-H) Small ribosomal subunit protein RPS6 and large ribosomal subunit protein RPL10L, and RNA-binding proteins MSI1 and TSN were relatively enriched in the XY body. (I-L) Translational activators MKNK1 and MKNK2, and repressors EIF4EBP1 and EIF2C4 (AGO4), were found in the XY body. Bars = 5 μm.

Article Snippet: 1:100 n.a Thermo EIF4E Mo Thermo MA1–089 AB_2536738 1:100 1:2000 EIF4E2 Mo Santa Cruz sc-100731 AB_1122491 1:100 n/a EIF4E3 Rab ProteinTech 17282–1-AP AB_2262162 1:100 n/a EIF4A1 Mo Thermo MA5–14901 AB_11006110 1:100 n/a EIF4A2 Rab ProteinTech 11280–1-AP AB_2097378 1:100 1:2000 EIF4A3 Rab Abcam Ab32485 AB_732124 1:50 n/a SYCP3 Rat Handel Lab. Eaker et al.,2001 n.a.

Techniques: RNA Binding Assay